Journal: Oncology Reports

Article Title: Expression level of Bcl-XL critically affects sensitivity of hepatocellular carcinoma cells to LIGHT-enhanced and interferon-γ-induced apoptosis

doi: 10.3892/or.17.5.1067

Figure Lengend Snippet: Figure 3. Expression levels of caspase-3, caspase-8, caspase-9, Bcl-XL, Bak and Bid in Hep3B cells treated with IFN-Á/LIGHT. (A) Hep3B cells were treated with 100 ng/ml IFN-Á and various concentrations of LIGHT for 24 h or 72 h as indicated. Cell lysates containing 30 μg of protein were subjected to 12% Tris-glycine gel electrophoresis followed by Western blotting with rabbit polyclonal antibodies against caspase-3, caspase-8, caspase-9, Bcl- XL, Bak, Bid, respectively. Cells treated with 100 ng/ml IFN-Á or 100 ng/ml LIGHT alone and untreated cells were used as the controls. ß-actin was used as an internal control for total protein loading. (B) Hep3B cells (2x105) treated with 100 ng/ml sLIGHT and 100 ng/ml of IFN-Á were also incubated with 100 μM of caspase-3 inhibitor Z-DEVD-fmk, caspase-9 inhibitor Z- IEHD-fmk, caspase-8 inhibitor Z-IEHD-fmk, or normal saline for 96 h. Cell viability was then measured. The viability of untreated (without any cytokine and inhibitor) cells was set at 100%, and the relative viability of other samples was calculated accordingly. The experiments were performed in triplicate, and in at least two separated experiments. The bar indicates the standard error. **p<0.01, versus group treated with normal saline.

Article Snippet: A recombinant LIGHT soluble protein that only contained the extracellular region of human LIGHT and caspase inhibitors Z-DEVD-fmk, Z-IETD-fmk and Z-LEHD-fmk was purchased from R&D System (Minneapolis, MN, USA).

Techniques: Expressing, Nucleic Acid Electrophoresis, Western Blot, Control, Incubation, Saline

Journal: Genetics Research

Article Title: Ultrasound-Targeted Microbubble Destruction-Mediated Inhibition of Livin Expression Accelerates Ovarian Cancer Cell Apoptosis

doi: 10.1155/2021/7624346

Figure Lengend Snippet: Western blots of Livin, caspase-3, and caspase-8 in OVCA-433 cells. No. 1, blank group; no. 2, shRNA-Livin group; no. 3, shRNA-Livin-NMB group; no. 4, shRNA-Livin-TMB group; no. 5, shRNA-Livin + US group; no. 6, shRNA-Livin-NMB + US group; no. 7, shRNA-Livin-TMB + US group.

Article Snippet: The PVDF membrane was sealed using 5% skimmed milk powder for 1 h, probed with anti-Livin antibody (ab236500, Abcam, Cambridge, UK), anti-caspase-3 antibody (ab32351, Abcam), anti-caspase-8 antibody (ab32397, Abcam), and anti-GAPDH antibody (ab8245, Abcam) overnight at 4°C, and then reprobed with secondary antibody IgG conjugated with HRP.

Techniques: Western Blot, shRNA